E. Coli: Shiga Toxin Methods and Protocols Hardcover - 2002
by Dana Philpott (Editor); Frank Ebel (Editor)
First line
Shiga toxin (Stx)-producing Escherichia coli (STEC), also referred to as Verocytotoxin-producing E. coli (VTEC) (1), are causes of a major, potentially fatal, zoonotic food-borne illness whose clinical spectrum includes non-specific diarrhea, hemorrhagic colitis, and the hemolytic uremic syndrome (HUS) (2-6).
From the rear cover
Shiga toxin-producing Escherichia coli (STEC) and its isolated cytotoxin (Stx), also known as "hamburger E. coli" are continuing sources of significant-and sometime fatal-human and animal infections. In E. coli: Shiga Toxin Methods and Protocols, a multidisciplinary panel of leading experimentalists details the key protocols for the diagnosis and study of STEC and Stx pathogenesis. Described in step-by-step detail, these readily reproducible techniques range from those for the diagnosis and detection of STEC bacteria in patient and animal samples, to those for studying the cellular microbiology of STEC infections, especially host-pathogen interactions and the hemolysin of STEC. There are also protocols for studying the details of Shiga toxin (Stx) biology-from the purification of the toxin to the effects of Stx on various host cell functions-and for exploring STEC-mediated disease in various animal models. Each protocol has been thoroughly tested and optimized to produce robust and successful experimental results, and includes troubleshooting tips, advice on avoiding pitfalls, and alternate methods.
Comprehensive and up-to-date, E. coli: Shiga Toxin Methods and Protocols offers bacteriologists and medical microbiologists today's most powerful tools for illuminating the molecular and cellular microbiology of STEC's bacterial pathogenesis, as well as the mechanisms of its toxicity.
Details
- Title E. Coli: Shiga Toxin Methods and Protocols
- Author Dana Philpott (Editor); Frank Ebel (Editor)
- Binding Hardcover
- Edition U. S. EDITION
- Pages 352
- Volumes 1
- Language ENG
- Publisher Humana, USA
- Date 2002-09-26
- Illustrated Yes
- ISBN 9780896039391 / 0896039390
- Weight 1.51 lbs (0.68 kg)
- Dimensions 9.21 x 6.14 x 0.81 in (23.39 x 15.60 x 2.06 cm)
- Library of Congress subjects Escherichia coli infections, Verocytotoxins
- Library of Congress Catalog Number 2002068800
- Dewey Decimal Code 616.92
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E Coli: Shiga Toxin Methods and Protocols
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