The Elisa Guidebook: Second Edition Paperback - 2010
by John R. Crowther
From the rear cover
The Enzyme Linked Immunosorbent Assay (ELISA) continues, as predicted, to be the predominant assay used in biomedical and agricultural research. While little has changed in the principles of the ELISAs used since the publication of The ELISA Guidebook, many technological advantages, including the increasing number of reagents available for exploitation and the larger spectrum of instruments to measure the coloured products of ELISAs, have greatly benefited the field. Other testing technologies using molecular techniques, predominantly the conventional and more and more often, real time PCR, are rapidly evolving to help diagnose and differentiate disease agents and identify substances present in exquisitely small amounts. The ELISA is still needed either alone in mass screening, such as serological assays, or used in tandem with molecular techniques to fully evaluate the molecular and antigenic nature of analytes.
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. This Edition enlarges on charting methods for assessing the indirect ELISA, ruggedness and robustness of tests, aspects of kit use and validation, and internal quality control and external quality management of data. The author describes each method in great detail to ensure experimental success and includes advice on equipment choice, maintenance, and calibration. Wherever possible, helpful written explanations are provided along with copious diagrams.
With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook, Second Edition offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply ELISA methodology successfully in day-to-day basic and clinical research.
Details
- Title The Elisa Guidebook: Second Edition
- Author John R. Crowther
- Binding Paperback
- Edition Softcover reprin
- Pages 566
- Volumes 1
- Language ENG
- Publisher Humana
- Date 2010-11-19
- Illustrated Yes
- Features Illustrated
- ISBN 9781617378843 / 1617378844
- Weight 2.55 lbs (1.16 kg)
- Dimensions 10.2 x 7.5 x 1.3 in (25.91 x 19.05 x 3.30 cm)
- Dewey Decimal Code 616.075
More Copies for Sale
The ELISA Guidebook
by John R. Crowther
- New
- Condition
- New
- ISBN 10 / ISBN 13
- 9781617378843 / 1617378844
- Quantity Available
- 897
- Seller
-
Uxbridge, Greater London, United Kingdom
- Item Price
-
SGD 233.77SGD 13.94 shipping to USA
Show Details
The Elisa Guidebook: Second Edition
by John R. Crowther
- New
- Paperback
- Condition
- New
- Binding
- Paperback
- ISBN 10 / ISBN 13
- 9781617378843 / 1617378844
- Quantity Available
- 2
- Seller
-
Exeter, Devon, United Kingdom
- Item Price
-
SGD 362.98SGD 17.45 shipping to USA
Show Details
The ELISA Guidebook
by John R. Crowther
- New
- Condition
- New
- ISBN 10 / ISBN 13
- 9781617378843 / 1617378844
- Quantity Available
- 4
- Seller
-
Woodside, New York, United States
- Item Price
-
SGD 490.53SGD 5.48 shipping to USA