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PCR Cloning Protocols
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PCR Cloning Protocols Hardcover - 2002

by Bing-Yuan Chen (Editor); Harry W. Janes (Editor)


From the publisher

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

Details

  • Title PCR Cloning Protocols
  • Author Bing-Yuan Chen (Editor); Harry W. Janes (Editor)
  • Binding Hardcover
  • Edition U. S. EDITION
  • Pages 439
  • Volumes 1
  • Language ENG
  • Publisher Humana Press
  • Date March 2002
  • Illustrated Yes
  • ISBN 9780896039735 / 0896039730
  • Weight 1.65 lbs (0.75 kg)
  • Dimensions 8.91 x 6.74 x 1.01 in (22.63 x 17.12 x 2.57 cm)
  • Library of Congress subjects Molecular cloning, Polymerase chain reaction
  • Library of Congress Catalog Number 2001039702
  • Dewey Decimal Code 572.86
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